Bio-Chemical Tests for the Detection of Amino Acids

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There are many tests for the detection of Amino Acids. The most fundamental tests are given below:

1. Millon’s Test

2. Ninhydrine Test

Millon's Test for Detecting Amino acids

Millon’s Test for Detecting Amino acids

Millon’s Test:

To the test solution add about 2 ml of Millons reagent, if white precipitate is formed, it indicates the presence of amino acids.

Composition of Millon’s Reagent:

The reagent consists of mercury in nitric acid and is diluted with water.

Ninhydrine Test:

To the test solution add ninhydrine solution, boil it, violet colour if formed indicates the presence of amino acids.

Composition of Ninhydrine Reagent:

Ninhydrine is chemically 2,2-dihydroxyindane-1,3-dione.

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